A reverse phase high performance liquid chromatography (RP-HPLC) method was developed for estimation of artesunate without the use of buffer solution in mobile phase. Isocratic elution was achieved by use of acetonitrile: methanol: water as mobile phase. A C18 column of dimension 250 mm x 4.6 mm, 5 �µ was used and the flow rate was maintained at 1.2 ml/min. Artesunate was detected using UV detector at 220 nm. The linearity was evaluated between concentrations 800 to 4000 �µg/ml. The method was validated further for specificity, accuracy and precision. Artesunate was subjected to acid hydrolysis, base hydrolysis and oxidation for carrying out forced degradation studies.
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